SUPPRESSION OF THE KILLER PHENOTYPE IN USTILAGO MAYDIS

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Specificity of Ustilago maydis killer proteins.

Bacteria and fungi were tested for sensitivity to Ustilago maydis killer strains carrying virus-like particles. Various species taxonomically related to U. maydis were sensitive.

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The virally encoded killer proteins from Ustilago maydis

Several strains of Ustilago maydis, a causal agent of corn smut disease, exhibit a ‘killer’ phenotype that is due to persistent infection by double-stranded RNA Totiviruses. These viruses produce potent killer proteins that are secreted by the host. This is a rare example of virus/host symbiosis in that these viruses are dependent upon host survival and, to that end, produce antifungal proteins...

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سیتولوژی Ustilago maydis

رفتار هسته در تلیوسپور، بازیدیوسپور و میسلیوم­های Ustilago maydis مورد بررسی قرار گرفت. جهت کشت تلیوسپورها از محیط PDA + 10% دکستروز استفاده گردید. مطالعه رفتار هسته با استفاده از میکروسکوپ اپی­فلورسنت و رنگ­آمیزی با دو رنگ اتیدیوم برماید و اکردین اورنج صورت پذیرفت. هسته در تلیوسپور کروی و بزرگ و اکثر فضای داخلی آن را اشغال نموده است. بازیدیوسپورها (اسپوریدیوم) به صورت غیرانگلی و به روش جوانه­ز...

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Glycolysis in Ustilago maydis.

The kinetic parameters of the 10 glycolytic enzymes and glycolytic fluxes were determined for the first time in Ustilago maydis. Enzyme activities in yeast grown in minimal medium and harvested in the stationary stage were twofold higher than those from yeast grown in rich medium. In contrast, in yeast harvested in the exponential stage, the enzyme activities were higher in cells grown in rich ...

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Killer Phenomenon in USTILAGO MAYDIS: The Organization of the Viral Genome.

The double-stranded RNA content, the production of inactive killer protein, and the presence of virus-like particles were examined in induced nonkiller mutants and nonkiller progeny from a cross between a killer strain and a sensitive strain. A correlation between the loss of the 0.7 x 10(6) daltons dsRNA of the Ustilago maydis P6 virus and the lack of synthesis of the killer protein was establ...

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ژورنال

عنوان ژورنال: Genetics

سال: 1976

ISSN: 1943-2631

DOI: 10.1093/genetics/82.4.629